human primary epidermal melanocyte cells  (ATCC)

Journal: ACS Nano

Article Title: Multiplexed Nanoscale Viscoelastic Mapping at Multiple Time Scales of Melanoma Cells as a Label-Free Cancer Biomarker

doi: 10.1021/acsnano.5c01873

Figure Lengend Snippet: Altered organization of filamentous actin and microtubule cytoskeletons in melanoma cancer cells. Maximum intensity Z-projections (XY) demonstrating alterations in filamentous actin (magenta) and microtubule cytoskeletons (green). Immunofluorescence images of cells were captured using a Zeiss LSM 900 Airyscan 2. Compared with benign counterpart fibroblasts and melanocytes, highly malignant melanoma cells exhibit significant decreases in F-actin and microtubule network density.

Article Snippet: Human primary epidermal melanocyte cells were obtained from ATCC (Cat. # PCS-200-013) and cultured in Dermal Cell Basal Medium (ATCC, Cat. # PCS-200-030) supplemented with Phenol Red (ATCC) and Adult Melanocyte Growth Kit (ATCC, Cat. # PCS-200-042).

Techniques: Immunofluorescence

Journal: Journal of Synchrotron Radiation

Article Title: Effects of synchrotron-based X-rays and gold nanoparticles on normal and cancer cell morphology and migration

doi: 10.1107/S1600577522012024

Figure Lengend Snippet: Human epidermal melanocytes HEM and human primary melanoma MM418-C1 monolayer 24 h after being exposed to 1000 Gy 90 kVp SBB (width 500 µm). Both cell types are treated with 1 m M AuNPs. The boundaries of the radiation fields are marked with orange lines. (A1) HEM immediately after exposure, (A2) 24 h, (A3) 48 h and (A4) 96 h after exposure. (B1) MM418-C1 immediately after exposure, (B1) 24 h, (B2) 48 h and (B4) 96 h after exposure.

Article Snippet: Moshi Geso), and human colorectal adenocarcinoma SW48 (ATCC ® CCL-231 TM ; Manassas, VA, USA) – along with two different normal cell lines – human epidermal melanocytes HEM (ATCC ® PCS-200–013 TM ; Manassas, VA, USA) and human primary colon epithelial CCD841 CoN (ATCC ® CRL-1790 TM ; Manassas, VA, USA).

Techniques:

Journal: Frontiers in Oncology

Article Title: Long Non-Coding RNA CD27-AS1-208 Facilitates Melanoma Progression by Activating STAT3 Pathway

doi: 10.3389/fonc.2021.818178

Figure Lengend Snippet: The expression of lncRNA CD27-AS1-208, which locates in the nucleus, is increased during melanoma progression and significantly correlation with Ki-67 level. (A) Validation of differentially-expressed lncRNAs with a new cohort of samples. (B) The expression level of CD27-AS1-208 in melanocytes and melanoma cell lines. (C, D) The expression level of CD27-AS1-208 in the cytoplasm and nucleus of melanoma cells. (E) The location of CD27-AS1-208 in primary melanoma and nevus. (F) Immunofluorescence staining of Ki-67 in indicated melanoma tissues. (G) Correlation between CD27-AS1-208 level and Ki67 staining in melanoma tissue. * P < 0.05, ** P < 0.01, *** P < 0.001, NHEM or cytoplasm as control; # HMS as control, # P < 0.05, ## P < 0.01; & PIG1 as control, & P < 0.05, && P < 0.01. Scale bar = 10μm. ns., not significant.

Article Snippet: The immortalized normal human epidermal melanocyte cell line PIG1 was a gift from Dr. Caroline Le Poole, Loyola University Chicago, Maywood, IL ( ).WM793B, WM35, A375, WM-266, A2058, 451Lu, Hs294T, 1205Lu, SK-MEL-1 and SK-MEL-5 were purchased from American Type Culture Collection (ATCC) in 2014.

Techniques: Expressing, Biomarker Discovery, Immunofluorescence, Staining, Control